Cas9-mediated gene disruption in tetraploid Giardia intestinalis
V. Horackova, L. Voleman, K. D. Hagen, M. Petru, M. Vinopalova, F. Weisz, N. Janowicz, L. Markova, A. Motyckova, P. Tumova, S. C. Dawson, P. Dolezal
This study demonstrates the successful use of CRISPR/Cas9 to disrupt multiple genes in Giardia intestinalis, achieving full knock-out of three genes and partial disruption of an essential gene.
bioRxiv · Apr 21, 2021 · PreprintCell Biology