M. Zaffagnini

Chloroplasts lacking class I glutaredoxins are functional but show a delayed recovery of protein cysteinyl redox state after oxidative challenge

The paper shows that removing class I GRX activity in the chloroplast stroma of P. patens delays protein cysteine redox recovery after oxidative stress without affecting photosynthesis or NPQ.

Structure of the Calvin-Benson-Bassham sedoheptulose-1,7-bisphosphatase SBPase from the model microalga Chlamydomonas reinhardtii

This study reports the first structure of SBPase from Chlamydomonas reinhardtii, revealing its topology, active site residues, and dynamic oligomeric states.

Structural and functional characterization of chloroplast ribulose-5-phosphate-3-epimerase from the model green microalga Chlamydomonas reinhardtii

This study characterizes the structural and functional properties of CrRPE1, an enzyme in the Calvin-Benson-Bassham cycle of Chlamydomonas reinhardtii, revealing its catalytic behavior and regulatory mechanisms.

Structural snapshots of nitrosoglutathione binding and reactivity underlying S-nitrosylation of photosynthetic GAPDH

This study investigates how GSNO induces S-nitrosylation of CrGAPA, revealing structural and mechanistic insights into its redox specificity and enzyme inactivation.

Application of the SMALP technology to the isolation of GPCRs from low-yielding cell lines

This paper optimizes SMALP protocols to improve GPCR isolation from low-yielding human cell lines by adjusting ionic strength and combining chromatography techniques.

Crystal structure of chloroplastic thioredoxin z defines a novel type-specific target recognition

The paper determines the crystal structure of chloroplastic thioredoxin z, revealing a unique electrostatic surface that enables specific recognition of target proteins.

Structural and functional insights into nitrosoglutathione reductase from Chlamydomonas reinhardtii

The paper characterizes CrGSNOR1 from Chlamydomonas reinhardtii, revealing its strict specificity for GSNO and NADH, conserved 3D structure, and resistance to thiol-based redox modifications.

Arabidopsis and Chlamydomonas phosphoribulokinase crystal structures complete the redox structural proteome of the Calvin-Benson cycle

This paper determines the crystal structures of phosphoribulokinase (PRK) from Arabidopsis and Chlamydomonas, revealing structural features related to TRX regulation in the Calvin-Benson cycle.

Glutathionylation primes soluble GAPDH for late collapse into insoluble aggregates

This study shows that glutathionylation of AtGAPC1 in Arabidopsis primes it for aggregation, leading to insoluble, inactive protein chains.