Defocused reflectance imaging for low numerical aperture, large field of view quantitative live cell imaging studies
SOPHIE BULLOCH, Tienan Xu, David Herrmann, Paul Timpson, Tri Giang Phan, Yu-Hsuan Lin, Makoto Banno, Yean Jin Lim, Woei Ming Lee
This paper demonstrates that controlled defocusing in low numerical aperture microscopes improves automated cell segmentation and tracking for high-throughput live-cell imaging.
bioRxiv · Mar 24, 2025 · PreprintBiophysics