Comprehensive cellular analysis with single-nucleus RNA-seq of archived PAXgene whole blood samples
Ojasvi Chaudhary, Mia K. Steinberg, Grant E. Duclos, Peter Gathungu, Manisha Rao, Rogelio Aguilar, Varsha Shankarappa, Chris M. Rands, Xi Chen, Rebecca Halpin, Elizabeth Galery, Joseph F. Boland, Maurizio Scaltriti, Brian Andrew Dougherty, Asaf Rotem
The paper develops a method for single-nucleus RNA-seq of archived PAXgene whole blood samples, achieving high nuclei yields and unbiased cell-type representation through optimized isolation and globin depletion.
Cell Reports Methods · Mar 1, 2026Peer-reviewedGenomics