Comprehensive cellular analysis with single-nucleus RNA-seq of archived PAXgene whole blood samples
Ojasvi Chaudhary, Mia K. Steinberg, Grant E. Duclos…·Asaf Rotem
The paper develops a method for single-nucleus RNA-seq of archived PAXgene whole blood samples, achieving high nuclei yields and unbiased cell-type representation through optimized isolation and globin depletion.