Target engagement studies and kinetic live-cell degradation assays enable the systematic characterization of HDAC6 PROTACs
Maria Hanl, Felix Feller, Irina Honin, Kathrin Tan, Martina Miranda, Linda Schäker-Hübner, Nico Bückreiß, Matthias Schiedel, Michael Gütschow, Gerd Bendas, Finn Kristian Hansen
This paper presents a NanoBRET assay to assess HDAC6 target engagement and establishes a system for kinetic live-cell degradation analysis of HDAC6 PROTACs.
bioRxiv · Mar 31, 2025 · PreprintPharmacology & Toxicology