Comprehensive assessment of multiple biases in small RNA sequencing reveals significant differences in the performance of widely used methods
Carrie Wright, Anandita Rajpurohit, Emily E. Burke, Courtney A. Williams, Leonardo Collado‐Torres, Martha Kimos, Nicholas J. Brandon, Alan J. Cross, Andrew E. Jaffe, Daniel R. Weinberger, Joo Heon Shin
This study evaluates biases in small RNA sequencing methods, showing that UMIs improve accuracy and highlighting limitations in current miRNA and isomiR quantification.
BMC Genomics · Jun 21, 2019Peer-reviewedGenomics