Oligonucleotide genetics for Pseudomonas aeruginosa enables high throughput hypomorph screening
Sundar Pandey, Ayesha M Ahmed, Kanna Nagamatsu, Melissa Reyes, Jiwoong Kim, Xiaowei Zhan, David E. Greenberg, Scott H. Saunders
This paper introduces ORBIT, an oligonucleotide-based genetic toolkit for Pseudomonas aeruginosa that enables rapid, precise, and high-throughput hypomorph screening of essential genes.
bioRxiv · May 20, 2026 · PreprintMicrobiology