Scott H. Saunders

This paper introduces ORBIT, an oligonucleotide-based genetic toolkit for Pseudomonas aeruginosa that enables rapid, precise, and high-throughput hypomorph screening of essential genes.

This study uses CRISPRi screening to identify E. coli genes affecting lycopene production, finding 31 genes that significantly modify yield across various metabolic pathways.

The paper identifies hundreds of unannotated translation products in E. coli using a high-throughput reverse genetic approach, including novel polypeptides and chimeric proteins.

The paper shows that sequential antibiotic treatment leads to convergent multidrug resistance through extinction of resistance mutations, with single-cell RNA sequencing revealing metabolic and translational state changes.