CRISPR-Hybrid: A CRISPR-Mediated Intracellular Directed Evolution Platform for RNA Aptamers
Qiwen Su-Tobon, Jiayi Fan, Michael Goldstein, Kevin A. Feeney, Hongyuan Ren, Patrick Autissier, Peiyi Wang, Yingzi Huang, Udayan Mohanty, Jia Niu
This paper introduces a CRISPR-mediated platform for intracellular directed evolution of RNA aptamers, identifying novel orthogonal aptamer-RBP pairs for multiplexed gene regulation.
Nature Communications · Jan 11, 2025Peer-reviewedSynthetic Biology