Michael S. Eisenstein

Rationally designed split Lettuce aptamer based on large scale mutational analysis

This paper identifies high-performance split sites in DNA aptamers through large-scale mutational analysis, improving signal-to-background ratio for RNA detection.

Aptamer charge-amplified field-effect transistor biosensors achieve picomolar detection limits for small-molecule biomarkers in complex biological matrices

This paper develops a charge-amplified FET biosensor for detecting small-molecule biomarkers with picomolar sensitivity in complex biological samples.

Peptide sequencing via reverse translation of peptides into DNA

This paper introduces a method to sequence peptides by converting them into DNA barcodes and demonstrates its ability to detect single amino acid substitutions and post-translational modifications.

Discovery of high-specificity DNA aptamers for progesterone using a high-throughput array platform

This paper develops high-specificity DNA aptamers for progesterone using a high-throughput array platform, achieving nanomolar affinity and minimal cross-reactivity with structurally similar steroids.

Accelerated Discovery of Aptamer Beacons via Massively Parallel Screening

This paper introduces MAPA, a high-throughput platform for discovering and optimizing aptamer beacons for molecular targets, demonstrating its effectiveness for glucose, serotonin, and dopamine.

A multiplexed and quantitative assay for small-molecule detection via photo-crosslinking of structure switching aptamers

The paper introduces xPlex, a method for multiplexed and quantitative detection of small-molecule analytes using aptamer switches and UV-crosslinkable strands, demonstrating its effectiveness with dopamine and glucose.

A direct selection strategy for isolating aptamers with pH-sensitive binding activity

This paper describes a method to isolate pH-sensitive aptamers through direct selection, demonstrating aptamers with switchable binding affinity at different pH levels.