Structural basis of underwound DNA topology bearing PAM-mutant recognition by AtCas9
Min Duan, Bing Meng, Lei Zhou, Lijie Wu, Xiaohan Tong, Dongchao Huang, Hao Yin, Zheng Liu, Ying Zhang
This study reveals how DNA topology affects PAM recognition by AtCas9 through cryo-EM structures of underwound DNA complexes.
bioRxiv · Jan 30, 2026 · PreprintBiochemistry