An optimised protocol for quality control of gene therapy vectors using Nanopore direct RNA sequencing
Kathleen Zeglinski, Christian Montellese, Matthew E. Ritchie, Monther Alhamdoosh, Cédric Vonarburg, Rory J. Bowden, Monika Jordi, Quentin A. Gouil, Florian Aeschimann, Arthur L. Hsu
This paper compares long-read sequencing technologies to identify RNA quality issues in lentiviral vectors, finding Nanopore direct RNA sequencing most effective for detecting incomplete RNA.
bioRxiv · Dec 3, 2023 · PreprintGenomics