Florian Aeschimann

An optimised protocol for quality control of gene therapy vectors using Nanopore direct RNA sequencing

This paper compares long-read sequencing technologies to identify RNA quality issues in lentiviral vectors, finding Nanopore direct RNA sequencing most effective for detecting incomplete RNA.

LSM2-8 and XRN-2 contribute to the silencing of H3K27me3-marked genes through targeted RNA decay

The paper identifies a pathway in C. elegans where LSM2-8 and XRN-2 selectively degrade H3K27me3-marked genes, revealing a new layer of heterochromatin regulation in animals.

Two parallel arms of the heterochronic pathway direct coordinated juvenile-to-adult transition through distinct LIN-29 isoforms

The paper identifies two LIN-29 isoforms that regulate distinct aspects of the juvenile-to-adult transition in C. elegans through differential spatiotemporal expression and regulatory control by LIN-28, LIN-41, and HBL-1.

let-7 controls the transition to adulthood by releasing select transcriptional regulators from repression by LIN41

The paper shows let-7 miRNA promotes adult development in C. elegans by releasing LIN41 repression of multiple transcriptional targets involved in sexual organ development and skin progenitor cell fate changes.

Timing mechanism of sexually dimorphic nervous system differentiation

The paper identifies a regulatory pathway controlling the timing of sexual differentiation in C. elegans and shows its conservation in mice and humans.