Author

N. Daffern

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 2 Bioengineering· 1

Signature Assays, Organisms & Methods

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Research Focus:protein folding & dynamics· 2 Research Focus:benchmarking· 1 Approach:deep learning· 1 Approach:experimental assay· 1 Approach:mechanistic / dynamical model· 1 Organism:plant (other)· 1 Assay:protein sequence· 1 Approach:statistical model· 1

Frequent Co-Authors

T. A. Whitehead· 2 shared I. M. Francino-Urdaniz· 1 shared I. Radhakrishnan· 1 shared I. Wheeldon· 1 shared J. Villegas· 1 shared J. Woojuh· 1 shared K. A. Lindorff-Larsen· 1 shared K. E. Johansson· 1 shared

Activity by Year

2023-2024 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 19 mutual co-author links

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2 shared papersBiochemistry

T. A. Whitehead

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Author profile →

3 papers

Recent·Most cited

GMMA can stabilize proteins across different functional constraints

N. Daffern, K. E. Johansson, Z. Baumer, N. Delbert-Robertson, J. Woojuh, M. A. Bedewitz, Z. Davis, I. Wheeldon, S. Cutler, K. A. Lindorff-Larsen, T. A. Whitehead

The paper identifies 5 stabilizing mutations for PYR1 variants using GMMA that increase thermal stability without affecting functionality across different ligand-binding capabilities.

bioRxiv · Jan 30, 2024Biochemistry

Prostaglandins as Candidate Ligands for a Per-ARNT-Sim (PAS) Domain of Steroid Receptor Coactivator 1 (SRC1)

N. Daffern, K. Kelley, J. Villegas, I. Radhakrishnan

The paper identifies prostaglandins as potential ligands for the PAS-B domain of SRC1, showing both non-covalent and covalent binding modes.

bioRxiv · Jul 13, 2023 · PreprintBiochemistry

Benchmarking cassette-based deep mutagenesis by Golden Gate assembly

N. Daffern, Z. T. Baumer, I. M. Francino-Urdaniz, T. A. Whitehead

The paper benchmarks a Golden Gate method for constructing large, deeply mutagenized protein libraries and demonstrates its scalability and effectiveness.

bioRxiv · Apr 13, 2023 · PreprintBioengineering