Targeted gene deletion with SpCas9 and multiple guide RNAs in Arabidopsis thaliana: four are better than two
J. Ordon, N. Kiel, D. Becker, C. Kretschmer, P. Schulze-Lefert, J. Stuttmann
This study shows that using two pairs of guide RNAs with SpCas9 increases chromosomal deletion frequency in Arabidopsis, and TREX2 co-expression enhances deletion size without increasing deletion frequency.
bioRxiv · Jan 10, 2023 · PreprintPlant Biology