High-throughput CRISPR live-cell imaging of low-frequency chromosomal events quantifies the latent efficiency of chromosome engineering
Xixun Hu, Yuichiro Iwamoto, Kyotaro Yamazaki, Nanami Kishima, Natsuko Otaki, Hitomaru Miyamoto, Yuichiro Miyaoka, Yasuhiro Kazuki, Sadao Ota
Hi-CRI quantifies low-frequency chromosomal events in living cells with a 0.01% detection limit, revealing pre-readout attrition in chromosomal transfer experiments.
bioRxiv · May 13, 2026 · PreprintBioengineering