Yuichiro Miyaoka

Multi-color droplet digital PCR assay enables allele-specific quantification of heterogeneous genome editing outcomes

The paper develops a multi-color ddPCR assay to quantitatively distinguish multiple edited alleles in genome editing outcomes, offering a faster and more scalable alternative to sequencing.

Derivation of human post-mitotic cardiomyocytes from tetraploid iPSCs

This study generates tetraploid iPSCs to produce more mature human cardiomyocyte-like cells with improved characteristics compared to conventional iPSC-derived cardiomyocytes.

Astrocytic and endothelial GLUT1 restoration improves brain glucose homeostasis in GLUT1 deficiency syndrome

This study shows that restoring GLUT1 in both astrocytes and endothelial cells improves glucose homeostasis and cognitive function in GLUT1 deficiency syndrome.

High-throughput CRISPR live-cell imaging of low-frequency chromosomal events quantifies the latent efficiency of chromosome engineering

Hi-CRI quantifies low-frequency chromosomal events in living cells with a 0.01% detection limit, revealing pre-readout attrition in chromosomal transfer experiments.

High-throughput robotic isolation of human iPS cell clones reveals frequent homozygous induction of identical genetic manipulations by CRISPR-Cas9

The paper develops a high-throughput method to isolate human iPS cell clones and finds that homozygous genetic edits are more common than heterozygous ones.

Granulin Loss of Function in Human Mature Brain Organoids Implicates Astrocytes in TDP-43 Pathology

The paper shows that loss of GRN in human brain organoids leads to TDP-43 pathology, driven by astrocytes, and can be rescued by progranulin.

Rapid, precise quantification of large DNA excisions and inversions by ddPCR

The paper introduces ddXR, a method to rapidly and accurately quantify DNA excisions and inversions without sequencing, revealing high rates of large excisions and low dependence on linear distance.