Genome editing in mammals using CRISPR type I-D nuclease
Keishi Osakabe, Naoki Wada, Tomoko Miyaji, Emi Murakami, Kazuya Marui, Risa Ueta, Ryosuke Hashimoto, Chihiro Abe-Hara, Bihe Kong, Kentaro Yano, Yuriko Osakabe
This study identifies Cas10d as a multi-functional nuclease in CRISPR type I-D system, enabling targeted mutagenesis in human cells with specific deletion and insertion capabilities.
Communications Biology · Nov 6, 2020Peer-reviewedMolecular Biology