An Enhanced Split Intein-Mediated Ligation (SIML) Platform for Rapid Discovery and Functional Screening of Circular Bacteriocins
Ester Sevillano, Mohamed El Bakkoury, Irene Lafuente, Nuria Peña, Cleopatra Collado, Luis M. Cintas, Estefanía Muñoz-Atienza, Philippe Gabant, Pablo E. Hernández, Juan Borrero
The paper introduces an enhanced SIML platform for synthesizing and screening circular bacteriocins, showing that intein choice and cyclization sites affect antimicrobial activity.
Frontiers in Bioengineering and Biotechnology · Sep 24, 2026Peer-reviewedSynthetic Biology