Efficient CRISPR/Cas9 gene ablation in uncultured naive mouse T cells for in vivo studies
S. Nüssing, I. G. House, C. J. Kearney, S. J. Vervoort, P. A. Beavis, J. Oliaro, R. W. Johnstone, J. A. Trapani, I. A. Parish
This paper demonstrates efficient CRISPR/Cas9 gene ablation in uncultured naive mouse T cells for in vivo studies, showing gene deletion occurs independently of T cell activation.
bioRxiv · Aug 9, 2019Immunology