BiochemistrybioRxivPreprint
Fluorogenic, sub-single-turnover monitoring of enzymatic reactions involving NAD(P)H provides a generalised platform for directed ultrahigh throughput evolution of biocatalysts in microdroplets
Matthew Penner, Oskar James Klein, Maximilian Gantz…·Florian Hollfelder
The paper develops a fluorogenic assay for NAD(P)H detection to enable high-throughput screening of biocatalysts in microdroplets, demonstrating improved sensitivity and applicability for glycosyl hydrolases and IREDs.
DOI 10.1101/2023.11.22.568356