Author

Oskar James Klein

University of CambridgeORCID 0000-0003-2213-8003Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Approach:deep learning· 1 Research Focus:drug response / drug discovery· 1 Approach:machine learning (classical)· 1 Assay:metagenomics· 1 Research Focus:protein design· 1 Research Focus:protein folding & dynamics· 1 Assay:proteomics· 1

Frequent Co-Authors

Florian HollfelderiD· 3 shared Friederike E. H. NintzeliD· 2 shared Matthew PenneriD· 2 shared Maximilian GantziD· 2 shared Anne-Cathrin Prowald· 1 shared Daniel Boros· 1 shared E.C. CampbelliD· 1 shared Elie Patel· 1 shared

Activity by Year

2023-2025 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 33 mutual co-author links

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3 shared papersBiochemistry

Florian HollfelderORCID

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3 papers

Recent·Most cited

BiochemistrybioRxivPreprintOct 30, 2025

A portable dynamic loop module enables phosphotriesterase function in cysteine-dependent hydrolases

J. David Schnettler, E.C. Campbell, Rashid Khashiev…·Florian Hollfelder

The paper identifies a novel phosphotriesterase from the DLH family and shows that loop flexibility influences activity, enabling engineering of higher activity in a human enzyme.

1 citation

metagenomicsexperimental assay

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Synthetic BiologybioRxivPreprintApr 8, 2024

Microdroplet screening rapidly profiles a biocatalyst to enable its AI-assisted engineering

Maximilian Gantz, Simon V. Mathis, Friederike E. H. Nintzel…·Florian Hollfelder

The paper combines microfluidic screening with machine learning to rapidly engineer biocatalysts, achieving 23-fold improvement in catalytic efficiency through sequence-function mapping.

18 citations

machine learning (classical)protein design

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BiochemistrybioRxivPreprintNov 22, 2023

Fluorogenic, sub-single-turnover monitoring of enzymatic reactions involving NAD(P)H provides a generalised platform for directed ultrahigh throughput evolution of biocatalysts in microdroplets

Matthew Penner, Oskar James Klein, Maximilian Gantz…·Florian Hollfelder

The paper develops a fluorogenic assay for NAD(P)H detection to enable high-throughput screening of biocatalysts in microdroplets, demonstrating improved sensitivity and applicability for glycosyl hydrolases and IREDs.

DOI 10.1101/2023.11.22.568356

proteomicsdeep learning

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