Minimal activation of the p53 DNA damage response by a modular cytosine base editor enables effective multiplexed gene knockout in induced pluripotent stem cells
Robert A. Blassberg, Olga Mielczarek, Jesse Stombaugh, Hanneke Okkenhaug, Alice A. Torres, Paul Russell, John J. Lambourne, Immacolata Porreca
This paper demonstrates a cytosine base editor that enables efficient and durable multiplexed gene knockout in iPSCs with minimal p53 activation and reduced DNA damage.
bioRxiv · Jun 5, 2025 · PreprintMolecular Biology