Impact Of Sequencing Depth And Read Length On Single Cell RNA Sequencing Data: Lessons From T Cells
Simone Rizzetto, Auda A. Eltahla, Peijie Lin, Rowena Anne Bull, Andrew R. Lloyd, Joshua W. K. Ho, Vanessa Venturi, Fabio Luciani
This study examines how sequencing depth and read length affect scRNA-seq data quality, finding that sufficient depth and length improve TCRβ reconstruction and gene expression accuracy in T cells.
Scientific Reports · Oct 2, 2017Peer-reviewedBioinformatics