Scalable, FACS-Free Genome-Wide Phenotypic Screening
Barbara Mair, Peter M. Aldridge, Randy Singh Atwal, Sanna N. Masud, Meng Zhang, David N. Philpott, Amy H.Y. Tong, Edward Hartley Sargent, Stéphane Angers, Jason Moffat, Shana O. Kelley
The paper presents a high-throughput CRISPR-Cas9 screening method for identifying CD47 modulators, identifying QPCTL as a key enzyme involved in its pyroglutamyl modification.
bioRxiv · Apr 19, 2019 · PreprintGenomics