RNA-Seq is not required to determine stable reference genes for qPCR normalization
Nirmal Kumar Sampathkumar, Venkat Krishnan Sundaram, Prakroothi S. Danthi, Rasha Barakat, Shiden Solomon, Mrityunjoy Mondal, Ivo Carre, Tatiana El Jalkh, Aïda Padilla-Ferrer, Julien Grenier, Charbel Massaad, Jacqueline C. Mitchell
This study shows that using a robust statistical method to select reference genes from randomly chosen candidates is as effective as selecting stable genes from RNA-Seq data for qPCR normalization.
PLoS Computational Biology · Feb 28, 2022Peer-reviewedMolecular Biology