Molecular BiologyPLoS Computational BiologyPeer-reviewed
RNA-Seq is not required to determine stable reference genes for qPCR normalization
Nirmal Kumar Sampathkumar, Venkat Krishnan Sundaram, Prakroothi S. Danthi…·Jacqueline C. Mitchell
This study shows that using a robust statistical method to select reference genes from randomly chosen candidates is as effective as selecting stable genes from RNA-Seq data for qPCR normalization.