Genetic Code Expansion for Site-Specific Encoding of a Switchable, Intrinsic Fluorophore-Quencher Pair to Monitor Protein Dynamics
Priyanda Giri, Yarra Venkatesh, Moriah H. Mathis, Christina M. Hurley, Chloe M. Jones, Olivier Ndogo Eteme, Zachary M. Hostetler, Richard B. Cooley, Rahul M. Kohli, Ryan A. Mehl, E. James Petersson
This paper introduces a genetic code expansion method to integrate a fluorophore-quencher pair into proteins for monitoring dynamics, demonstrating applications in studying protein function and drug screening.
bioRxiv · Jun 29, 2026 · PreprintBiochemistry