Rahul M. Kohli

This paper introduces a genetic code expansion method to integrate a fluorophore-quencher pair into proteins for monitoring dynamics, demonstrating applications in studying protein function and drug screening.

The paper develops and validates Sparse-Seq, a low-coverage sequencing method for accurate quantification of 5mC and 5hmC levels, showing it is more accessible and less variable than mass spectrometry.

The paper introduces an unnatural DNA base modification to map protein-DNA occupancy in non-CpG contexts, revealing binding patterns of LexA in bacterial promoters.

This study shows TET1's non-catalytic activity helps maintain chromatin structure to prevent abnormal methylation in male germ cells.

The paper introduces MSA, a new epigenotyping platform for analyzing ternary-code DNA methylation, and applies it to uncover aging dynamics and trait associations involving 5hmC and 5mC.

This paper introduces modFDR, a framework to assess nanopore sequencing for DNA modification detection, showing it reliably detects abundant modifications but has high false positives for rare ones.

The paper develops a method to generate variant libraries in vivo using base editing to study gene regulatory programs in multicellular organisms.

The paper develops low-input and single-cell methods for Infinium DNA methylation BeadChips, improving detection rates and data quality for small sample sizes.

The paper identifies optimal RNA base editors for RBP-RNA interaction studies by evaluating over thirty rBEs using PRINTER framework.

This study develops snhmC-seq to quantify 5hmC in single cells and identifies non-canonical gene regulation by non-CG 5hmC in mouse brain.

This study shows that TET1's catalytic activity is essential for reprogramming imprinting control regions and sperm-specific hypomethylated regions during germline development.