Site-specific phosphorylation of Huntingtin exon 1 recombinant proteins enabled by the discovery of novel kinases
A. Chiki, J. Ricci, R. N. Hegde, L. A. Abriata, A. Reif, H. A. Lashuel
This paper introduces a method to produce site-specifically phosphorylated Httex1 proteins using novel kinases and SUMO-fusion purification, enabling further study of phosphorylation's role in Httex1 function.
bioRxiv · Jul 23, 2020Biochemistry