Real-time observation of DNA target interrogation and product release by the RNA-guided endonuclease CRISPR Cpf1
D. Singh, J. Mallon, A. Poddar, Y. Wang, R. Tipanna, O. Yang, S. Bailey, T. Ha
This study characterizes the DNA interrogation and product release kinetics of three Cpf1 orthologues using single-molecule fluorescence and biochemical assays, revealing differences from Cas9 in binding and cleavage requirements.
bioRxiv · Apr 9, 2018Biophysics