T. Ha

MARK2/MARK3 kinases are catalytic co-dependencies of YAP/TAZ in human cancer

This study identifies MARK2/3 as essential kinases for YAP/TAZ function in cancer and shows their inhibition can restore Hippo pathway tumor suppression.

Single molecule studies of the native hair cell mechanosensory transduction complex

This study optimizes single molecule assays to detect and quantify the native hair cell mechanosensory transduction complex at attomole levels.

Rapid long-distance migration of RPA on single stranded DNA occurs through intersegmental transfer utilizing multivalent interactions

This study investigates RPA's long-distance migration on ssDNA using biophysical methods, finding that intersegmental transfer via multivalent interactions enables rapid diffusion under tension.

Electrostatic encoding of genome organization principles within single native nucleosomes

This study shows that single nucleosomes encode A/B compartment information through biophysical properties, specifically condensability, which correlates with gene expression and is electrostatic in nature.

Dynamin 1xA interacts with Endophilin A1 via its spliced long C-terminus for ultrafast endocytosis

This study identifies a phospho-regulated interaction between Dyn1xA and Endophilin A1 via a unique C-terminal extension, essential for ultrafast endocytosis.

GAGA zinc finger transcription factor searches chromatin by 1D-3D facilitated diffusion

This study tracks GAF's chromatin search mechanism using smFRET and imaging, showing 1D-3D diffusion enables efficient target finding despite nucleosome barriers.

Dynamic 1D Search and Processive Nucleosome Translocations by RSC and ISW2 Chromatin Remodelers

This study investigates the dynamic search and processive nucleosome translocation mechanisms of RSC and ISW2 chromatin remodelers using optical tweezers and single-particle imaging.

A tubulin-binding protein that preferentially binds to GDP-tubulin and promotes GTP exchange

The paper identifies BuGZ as a tubulin GEF that binds GDP-tubulin and promotes GTP exchange, challenging the belief that such a factor is unnecessary.

Fluorescence lifetime analysis of smFRET with contribution of PIFE on donor and acceptor

This paper develops a Bayesian framework to analyze smFRET data and separate PIFE effects from FRET efficiency changes using fluorescence lifetime signals.

Linking folding dynamics and function of SAM/SAH riboswitches at the single molecule level

This study uses single-molecule FRET to analyze the conformational dynamics of SAM/SAH riboswitches, showing how ligand binding and folding kinetics regulate ribosome accessibility.

Massively parallel single molecule tracking of sequence-dependent DNA mismatch repair in vivo

The paper tracks DNA mismatch repair in E. coli using high-throughput sequencing and finds sequence-dependent repair efficiencies, with CC mismatches being poorly repaired and local context affecting TT, AG, and CT mismatches.

Dimensional Reduction for Single Molecule Imaging of DNA and Nucleosome Condensation by Polyamines, HP1α and Ki-67

This paper uses single-molecule imaging to study DNA and nucleosome condensation by polyamines, HP1α, and Ki-67, revealing kinetic differences in condensation and dissolution.

Sniper2L, a high-fidelity Cas9 variant with high activity

This paper develops Sniper2L, a high-fidelity Cas9 variant with improved specificity and retained high on-target activity, and introduces a deep-learning model to predict its activity.

Dependence of Nucleosome Mechanical Stability on DNA Mismatches

This study investigates how DNA mismatches affect nucleosome mechanical stability and DNA exposure using single-molecule techniques, finding that C-C mismatches increase stability and reduce accessibility.

Improving the sensitivity of in vivo CRISPR off-target detection with DISCOVER-Seq+

DISCOVER-Seq+ improves CRISPR off-target detection sensitivity by using DNA-PKcs inhibition and ChIP-seq to identify more off-target sites in cells and mice.

The START domain potentiates HD-ZIPIII transcriptional activity

This study identifies the START domain's role in enhancing HD-ZIPIII transcriptional activity through homodimerization and ligand-induced conformational changes.

Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins

This study evaluates the reliability and accuracy of single-molecule FRET for measuring protein structural dynamics and distances, finding high precision and accuracy with specific uncertainty limits.

Membrane compression by synaptic vesicle exocytosis triggers ultrafast endocytosis

The paper shows that actin filaments and membrane compression from synaptic vesicle exocytosis drive ultrafast endocytosis at synapses.

Subtype-specific single β1 integrin mechanics for activation, mechanotransduction and cytoskeleton remodeling

The paper investigates subtype-specific mechanics of β1 integrins in cell spreading, showing different tension thresholds and cytoskeletal remodeling for integrin α4β1 versus RGD-binding integrins.

Nucleosome sliding by the Chd1 chromatin remodeler relies on theintegrity of the DNA duplex

The paper investigates how the Chd1 chromatin remodeler is affected by DNA duplex integrity, finding that it is sensitive to poly(dA:dT) tracts and DNA mismatches.

Amplification of Olfactory Signals by Anoctamin 9 is Essential for Mammalian Olfaction: a Risk Factor for the Covid-19-associated Anosmia

This study identifies Anoctamin 9 as essential for mammalian olfaction, showing its role in amplifying olfactory signals and linking its downregulation to Covid-19-associated anosmia.

Sequence-dependent mechanochemical coupling of helicase translocation and unwinding at single-nucleotide resolution.

This study uses SPRNT to analyze how DNA sequence affects PcrA helicase kinetics during translocation and unwinding at single-nucleotide resolution.

Rtt105 configurationally staples RPA and blocks facilitated exchange and interactions with RPA-interacting proteins

This study shows Rtt105 binds to RPA, staples it, and blocks RIP interactions without ssDNA, while stabilizing RPA-ssDNA filaments with ssDNA.

ATP binding facilitates target search of SWR1 chromatin remodeler by promoting one-dimensional diffusion on DNA

This study shows ATP binding enables SWR1 chromatin remodeler to diffuse along DNA, facilitating efficient target search by sliding over DNA until encountering roadblocks.

Massively parallel genomic perturbations with multi-target CRISPR reveal new insights on Cas9 activity and DNA damage responses at endogenous sites

This paper introduces multi-target gRNAs (mgRNAs) to enable high-throughput CRISPR screening, revealing insights into Cas9 dynamics, DNA damage responses, and chromatin changes at endogenous sites.

Senescent stroma induces nuclear deformations in cancer cells via the inhibition of RhoA/ROCK/myosin II-based cytoskeletal tension

This study shows senescent stroma reduces cancer cell nuclear tension via RhoA/ROCK/myosin II inhibition, leading to morphological changes linked to malignancy.

Coordinated DNA and Histone Dynamics Drive Accurate Histone H2A.Z Exchange

The paper uses single-molecule FRET to show SWR1 unwraps DNA and exchanges H2A-H2B for H2A.Z-H2B with timed precision.

Patient-derived triple negative breast cancer organoids provide robust model systems that recapitulate tumor intrinsic characteristics

This study develops and characterizes patient-derived TNBC organoids that recapitulate tumor properties and identify key signaling pathways involved in cancer progression.

The repeat region of the circumsporozoite protein is an elastic linear spring with a functional role in Plasmodium sporozoite motility

The study identifies the CSP repeat region as a linear spring critical for sporozoite motility and adhesion site formation in malaria parasites.

A viral genome packaging ring-ATPase is a flexibly coordinated pentamer

The paper determines the composition of the bacteriophage T4 DNA packaging motor and shows that it can tolerate an inactive subunit, challenging the idea of strict coordination among motor subunits.