Efficient target cleavage by Type V Cas12a effector programmed with split CRISPR RNA
Regina Tkach, Natalia Nikitchina, Nikita Shebanov, Vladimir M. Mekler, Egor A. Ulashchik, Olga L. Sharko, Vadim V. Shmanai, Ivan Tarassov, Konstantin Severinov, Nina Entelis, Ilya Mazunin
This study shows that AsCas12a can efficiently cleave target DNA using split crRNAs, with residual activity even when only the spacer is present.
bioRxiv · Dec 21, 2020 · PreprintMolecular Biology