Nina Entelis

Modified crRNA variants for precise targeting of point mutations in human mitochondrial DNA by CRISPR-Cas12a

This study identifies modified crRNA variants for precise targeting of point mutations in human mitochondrial DNA using CRISPR-Cas12a, showing effective discrimination and base editing in vitro and in human cells.

Mitochondrial genome microhomology-mediated editing by donor DNA delivery into mitochondria in human cells.

This paper introduces a method for editing mitochondrial DNA using donor DNA and microhomology-mediated end joining, achieving targeted nucleotide changes in human cells.

Targeted deletions in human mitochondrial DNA engineered by Type V CRISPR-Cas12a system

This paper demonstrates the use of a Type V CRISPR-Cas12a system to induce targeted deletions in human mitochondrial DNA, offering a potential therapeutic approach for mitochondrial disorders.

Efficient target cleavage by Type V Cas12a effector programmed with split CRISPR RNA

This study shows that AsCas12a can efficiently cleave target DNA using split crRNAs, with residual activity even when only the spacer is present.

YBEY is an essential biogenesis factor for mitochondrial ribosomes

This study shows that human YBEY is essential for mitochondrial ribosome biogenesis, leading to respiratory deficiency and mitochondrial abnormalities.