Analysis of the ARTIC version 3 and version 4 SARS-CoV-2 primers and their impact on the detection of the G142D amino acid substitution in the spike protein
James J. Davis, Scott Wesley Long, Paul A. Christensen, Randall J. Olsen, Robert Olson, Maulik Shukla, Sishir Subedi, Rick Stevens, James M. Musser
The paper compares ARTIC V3 and V4 primer sets for SARS-CoV-2 sequencing, finding improved performance and underrepresentation of G142D substitution in early Delta genomes due to V3 primer limitations.
Microbiology Spectrum · Dec 8, 2021Peer-reviewedBioinformatics