CRISPR/dCas-mediated counter-silencing: Reprogramming dCas proteins into antagonists of xenogeneic silencers
Johanna Wiechert, Biel Badia Roigé, Doris Dohmen-Olma, Hindra Hindra, Xiafei Zhang, Roberto G. Stella, Marie A. Elliot, Julia Frunzke
This paper introduces CRISPRcosi, a CRISPR/dCas-mediated method to counteract xenogeneic silencers in actinobacteria, achieving gene activation without promoter engineering.
mBio · May 28, 2025Peer-reviewedMicrobiology