Off-target glycans encountered along the synthetic biology route towards humanized N-glycans in Pichia pastoris
Bram Laukens, Pieter P. Jacobs, Katelijne Geysens, José C. Martins, Charlot De Wachter, Paul Ameloot, Willy Morelle, Jurgen Haustraete, Jean‐Christophe Renauld, Bart Samyn, Roland Contreras, Simon Devos, Nico Callewaert
The paper identifies off-target glycans formed during the engineering of Pichia pastoris to produce humanized N-glycans, highlighting challenges in achieving precise glycosylation control.
Biotechnology and Bioengineering · May 6, 2020Peer-reviewedSynthetic Biology