Nico Callewaert

This study identifies GCNT2 as a glycosylation-dependent checkpoint that restrains intestinal intra-epithelial lymphocyte (IEL) activation by modifying CD45 and dampening TCR signaling.

The paper shows that low DMSO concentrations skew T cell differentiation towards a memory phenotype during in vitro expansion.

This study develops and tests dried food-formulated yeast-secreted VHH antibodies as prophylaxis against Clostridioides difficile disease in mice, showing high efficacy in preventing mortality and disease.

The paper introduces cf-RRBS, a method for methylation profiling of fragmented plasma DNA, enabling cost-effective and accurate non-invasive lung cancer subtyping.

This paper presents a yeast-based platform for producing stable and potent heavy chain-only antibodies, demonstrating its effectiveness with an anti-SARS-CoV-2 example.

This study identifies optimal sites for introducing N-glycans on VHH antibodies to enhance macrophage delivery, demonstrating glycan-dependent uptake in vitro and in vivo.

The paper develops an open-source yeast chassis, OPENPichia, for synthetic biology by reverse engineering and characterizing industrial Pichia strains.

This study evaluates PTM predictors using protein language models, revealing a training bias from proteomics data that overestimates prediction accuracy and suggests a solution through negative sample filtering.

The paper develops SECRiFY, a method to assess secretability of protein fragments in yeast, revealing intrinsic disorder and flexibility as key factors.

The paper describes the development of a broadly neutralizing antibody, XVR011, effective against SARS-CoV-2 and its variants in animal models.

This study demonstrates that reduced representation bisulfite sequencing of cell-free DNA can accurately classify pediatric solid tumors with high sensitivity.

The paper identifies off-target glycans formed during the engineering of Pichia pastoris to produce humanized N-glycans, highlighting challenges in achieving precise glycosylation control.