Development and validation of inducible protein degradation and quantitative phosphoproteomics to identify kinase-substrate relationships.
Rufus Hards, Charles L. Howarth, Kwame Wiredu, Ian S. LaCroix, Juan Mercado del Valle, Mark E. Adamo, Arminja Nadine Kettenbach, Andrew Jon Holland, Scott A. Gerber
The paper develops a method using inducible protein degradation and phosphoproteomics to identify kinase-substrate relationships, showing high selectivity and correlation with chemical inhibition.
bioRxiv · Dec 8, 2021 · PreprintSystems Biology