Repurposing the Streptococcus mutans CRISPR-Cas9 System to Understand Essential Gene Function
R. C. Shields, A. R. Walker, N. Maricic, B. Chakraborty, S. A. Underhill, R. A. Burne
The paper develops a CRISPR interference tool for Streptococcus mutans to study essential genes, identifying growth defects and phenotypes in 77% of targeted strains.
PLOS Pathogens · Mar 9, 2020Peer-reviewedMicrobiology