Impact of Single Freeze-Thaw Cycles on Serum Protein Stability: Implications for Clinical Biomarker Validation Using Mass Spectrometry
Thorben Sauer, Martina Oberländer, Regina Maushagen, H. Radloff, Rüdiger Braun, Kim C. Honselmann, Ulrich Friedrich Wellner, J. Habermann, Claudia Benecke, Tobias Keck, S. Szymczak, T. Gemoll
This study assesses the impact of a single freeze-thaw cycle on serum protein stability using mass spectrometry, finding significant abundance changes for 30 proteins, with 11 validated as potential biomarkers for pancreatic disease.
bioRxiv · Apr 9, 2025 · PreprintMolecular Biology