DNA-free CRISPR-Cas9 gene editing of tetraploid tomatoes using protoplast regeneration
C.-T. Hsu, Y.-H. Yuan, P.-X. Zheng, F.-H. Wu, Q.-W. Cheng, Y.-L. Wu, S. Lin, J.-J. Yue, Y.-H. Cheng, S.-I. L. Lin, M.-C. Shih, J. Sheen, Y.-C. Lin, C.-S. Lin
DNA-free CRISPR-Cas9 editing in wild tomatoes generates stable, inheritable diploid and tetraploid regenerants without chromosomal changes or off-target effects.
bioRxiv · Nov 2, 2021 · PreprintPlant Biology