Cas4 nucleases can effect specific integration of CRISPR spacers
Zhufeng Zhang, Saifu Pan, Tao Liu, Yingjun Li, Nan Peng
This study identifies Cas4 and Csa1 proteins as essential for specific CRISPR spacer acquisition, showing they recognize protospacer motifs and determine spacer length and orientation, with virus-encoded Cas4 proteins potentially inhibiting CRISPR-Cas interference.
Journal of Bacteriology · Mar 29, 2019Peer-reviewedMolecular Biology