Nan Peng

This study reprograms an archaeal TnpB endonuclease for genome editing, showing it can efficiently edit bacterial cells.

This study shows that Zymomonas mobilis uses primed CRISPR acquisition for specific invader DNA uptake and microhomology-mediated end-joining for repairing DNA breaks caused by self-targeted spacers.

This study identifies Cas4 and Csa1 proteins as essential for specific CRISPR spacer acquisition, showing they recognize protospacer motifs and determine spacer length and orientation, with virus-encoded Cas4 proteins potentially inhibiting CRISPR-Cas interference.