A shotgun approach for highly multiplexed mammalian metabolic engineering
Julie Trolle, Sara Sessa, Tori C. Rodrick, Drew R. Jones, David Fenyö, Sudarshan Pinglay, Jef D. Boeke
This paper introduces SGE, a method for screening metabolic pathways in mammalian cells, and finds that mitochondrial localization and large synthetic DNA constructs are key for successful pathway engineering.
bioRxiv · Jul 8, 2025 · PreprintSynthetic Biology