Synthesizing unmodified, supercoiled circular DNA molecules in vitro
Sepideh Rezaei, Monica Moncada-Restrepo, Sophia Leng, Jeremy W. Chambers, Fenfei Leng
This paper develops two biochemical methods to synthesize unmodified supercoiled circular DNA, demonstrating higher transfection efficiency in cells compared to bacterial-derived plasmids.
bioRxiv · Jan 24, 2025 · PreprintBiochemistry