Fenfei Leng

The paper identifies a potential hinge region in MtbTOP1 critical for conformational changes during DNA relaxation, showing that mutations disrupt enzyme activity.

This study shows that chromosomal topological domains in E. coli repress transcription, reduce orientation effects, and modulate gene coupling through supercoiling changes.

This paper develops two biochemical methods to synthesize unmodified supercoiled circular DNA, demonstrating higher transfection efficiency in cells compared to bacterial-derived plasmids.