Direct observation of conformational dynamics in intrinsically disordered proteins at the single-molecule level
Saaman Zargarbashi, Cyril Dominguez, Matthew Peters, Arman Yousefi, Sharon Munday, Yanhong Wang, Shreyasi Chatterjee, Andrew J. Hudson, Reuven Gordon, Christopher J. Mellor, Lei Xu, Mohsen Rahmani, Cuifeng Ying
This paper observes conformational dynamics of unmodified IDPs/IDRs at the single-molecule level using nanoaperture optical tweezers, revealing increased conformational variation and phosphorylation-induced compaction.
bioRxiv · May 26, 2025 · PreprintBiophysics