Verification of genetic engineering in yeasts with nanopore whole genome sequencing
Joseph H. Collins, Kevin W. Keating, Trent R. Jones, Shravani Balaji, Celeste B. Marsan, Marina Çomo, Zachary J. Newlon, Tom Mitchell, Bryan Bartley, Aaron Adler, Nicholas Roehner, Eric M. Young
The paper introduces Prymetime, a workflow for accurate whole genome sequencing of engineered yeasts, showing it can assemble and annotate synthetic parts and detect unintended modifications.
bioRxiv · May 5, 2020 · PreprintSynthetic Biology